(i
, MAG2) during CAP biotransformation and especially the expression of capO in the main CAP degrader Sphingomonas sp
The pLysS presence allows tight control of expression
New work reveals the structural basis of context-specific action of the
3
This protocol can be widely applied to recombinant protein expression for
Because T7 RNA
The ability to express and purify the desired recombinant protein in a large
Nat
Induce protein expression with best expresses your protein, you can use the glycerol stock to perform additional expression experiments
Vectors encoding resistance to kanamycin or chloramphenicol are now widely used as well
Chem
A resistant variant able to grow on plates containing 64 microg/ml chloramphenicol was obtained from the Enterobacter aerogenes ATCC 13048-type strain
1574-6968
0, 100 mM NaCl, 5 mM imidazole, 1% Triton X Autoinduction is a simple approach for protein expression that needs little user intervention after inoculation of the culture (Studier, 2005)
Here, we provide mechanistic insights into high-level resistance to chloramphenicol in C
Various strategies have been employed to improve and increase protein expression in E
Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme ( EC 2
The protein synthesis inhibitor chloramphenicol prevents the induction of KaiC and blocks phase shifting
Fine tuning of T7 expression can alleviate inclusion body formation or growth inhibitory effects from toxic proteins
Expression vectors can drive expression in various cell types (mammalian, yeast, bacterial, etc
We first expressed GFP-lunasin, a fusion protein that, because of its toxicity, has been impossible to produce in whole cells
The BL21(DE3) competent cells are an all-purpose strain for high-level protein expression and easy induction
pFN6K (Promega, Madison WI) and pET32 For analysis of protein expression, the PCR plates of frozen cell cultures were thawed and mixed with lysis buffer to obtain a final BL21 Competent E
In an indirect ELISA against 50 ng of BSA‐chloramphenicol, Chl‐VHH had an IC 50 concentration of 0
However, the function of AdpASv, an AdpA ortholog of Streptomyces venezuelae, is unknown